Fig. 2: Anatomical regulation of lymphocyte energetic resources.

A–C Immune cell populations were isolated from blood and lymph nodes of Vav-Cre/Perceval^fl/fl (SPICY) mice, and metabolic parameters were addressed by SPICE-Met flow cytometry. A Experimental scheme. B Heatmaps representing the net contribution (dependency) of OXPHOS, glycolysis, glucose and FA/AA for the indicated cell population. C Representative FACS profile showing Perceval-ATP and Perceval-ADP fluorescence and ATP:ADP ratio in CD8⁺ effector/effector memory T cells and CD8⁺ central memory T cells (left) and quantification of ATP:ADP ratio in the indicated populations (right). D–F Vav-Cre/Perceval^fl/fl (SPICY) mice were injected with MVA-vaccine and 7 days later, tetramer-positive cells were obtained from blood and lymph nodes, and their metabolic parameters were addressed. D Experimental scheme. E Representative FACS profile showing ATP and ADP fluorescence and ATP:ADP ratio in MVA-specific CD8⁺ effector/effector memory-like T cells and MVA-specific CD8⁺ central memory-like T cells. F Heatmaps and quantification of metabolic parameters of MVA-specific CD8⁺ effector/effector memory T cells and MVA-specific CD8⁺ central memory T cells. Compiled from 2 independent experiments with a total of 6 mice. Mean values ± SEM are shown. Statistical analyses were performed using a two-sided Wilcoxon test (C, F).