Fig. 3: Tissue microenvironment dictates T cell energetic charge.
From: Spatiotemporal regulation of energetic charge dictates T cell function
A Experimental scheme. Total immune cell populations from lymph nodes of Vav-Cre/Percevalfl/fl (SPICY) mice and injected into CD45.1 recipients. CD45.2-positive cells were isolated 3 h later from the blood and lymph nodes of recipient mice and their metabolic parameters were addressed. B Detection of CD45.2-positive cells isolated from CD45.1 recipients 3 h after the adoptive transfer. C Representative FACS plot showing the ATP and ADP fluorescence and ATP:ADP ratio in the indicated populations within CD45.2-positive cells detected in the blood and lymph nodes of recipient mice. D Heatmaps and quantification of energetic resources and metabolic parameters in the indicated populations within CD45.2-positive cells detected in the blood and lymph nodes of recipient mice. Mean values ± SEM are shown. Compiled from 3 independent experiments with a total of 6 recipient mice. Statistical analyses were performed using a two-sided Wilcoxon test.
