Fig. 7: Anti-tumor efficacy of PEF ablation, MOF-Fe, and M-C20U4V in KPC-A719 tumor models.
From: Disruption of iron homeostasis sensitizes pancreatic cancer to irreversible electroporation
a Schematic illustration of PEG-PCL-PB synthesis and M-C20U4V. b, c Representative histograms of hydrodynamic diameter (b) and surface ζ-potential (c) of M-C20U4V. Experiments were independently repeated three times with similar results. d Profiles of payload release from M-C20U4V, n = 3 independent experiments. e Immunoblots and quantification of FTH1 in Panc-1 cells treated with MOF-Fe and M-C20U4, n = 3 independent experiments. f, g Quantification of Panc-1 colonies (f) and HSA synergy scores (g) after treatment with MOF-Fe or M-C20U4V (in C20U4V-equivalent concentration), n = 3 independent experiments. h, i, Biodistribution of DiR-labeled M-C20U4V in KPC-A719 tumors (h). Micelles were intravenously injected 4 h prior to PEF ablation, and the tumor and major organs were collected at 44 h after PEF ablation, n = 5 (untreated) or 7 (PEF ablation) independent mice. Representative fluorescence images (i) of major organs and tumors include: heart (He), liver (Li), spleen (Sp), lung (Lu), kidneys (Ki), stomach (St), intestines (In), bone (Bo), muscle (Mu), and tumor (Tu). j Tumor growth curves of subcutaneous KPC-A719 models. n = 6 (sham control, PEF, MOF-Fe + M-C20U4V) or 14 (PEF ablation + MOF-Fe + M-C20U4V) independent mice. k Animal survival of orthotopic KPC-A719 models, n = 6 (sham control, MOF-Fe + M-C20U4V), 7 (PEF ablation), or 13 (PEF ablation + MOF-Fe + M-C20U4V) independent mice. Data were presented as mean ± SEM. Statistical significance was determined using one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison (e, j) or log-rank test (k), and p values are indicated. Source data are provided as a Source Data file. The micelle schemes in Fig.7a were generated using PowerPoint.
