Fig. 4: Further optimization of MmeFz2 via ssDBD fusion and validation of genome-editing efficiency at endogenous loci in mammalian cells.
a Genome-editing efficiencies of evoMmeFz2 with N- or C-terminal ssDBD and exonuclease fusions at five loci in HEK293T cells. b Comparison of average genome-editing efficiencies of 16 evoMmeFz2 variants across five endogenous loci in HEK293T cells. c Comparison of genome-editing efficiencies induced by WT-MmeFz2, enMmeFz2, enMmeFz2-HMG-D, evoMmeFz2, and evoMmeFz2-HMG-D at 38 endogenous loci in HEK293T cells. Data represent mean ± s.e.m. of three independent biological replicates. d The summary dot plot compares the activities of these five variants in HEK293T cells. P values were calculated using an unpaired two-tailed Student’s t test, with adjusted P (Padj) values of 7 × 10−8, 1 × 10−9, 7 × 10−9, and 9 × 10−10, respectively. For b and d each dot represents the mean editing efficiency of three independent biological replicates per endogenous locus. All mCherry-positive cells were FACS sorted to assess the MmeFz2-ωRNA editing efficiency. Source data are provided as a Source Data file.
