Fig. 5: Specificity of evoMmeFz2-mediated genome editing in mammalian cells. | Nature Communications

Fig. 5: Specificity of evoMmeFz2-mediated genome editing in mammalian cells.

From: Engineering the MmeFz2-ωRNA system for efficient genome editing through an integrated computational-experimental framework

Fig. 5: Specificity of evoMmeFz2-mediated genome editing in mammalian cells.

On- and off-target analyses of evoMmeFz2, evoMmeFz2-HMG-D, and WT-MmeFz2 were performed at six genomic loci (KRAS-guide1, CXCR4-guide2, DYRK1A-guide1, B2M-guide1, B2M-guide2, and B2M-guide5), with off-target sites containing 2 to 5 mismatches identified by Cas-OFFinder. Values are expressed as the mean of three independent biological replicates. Source data are provided as a Source Data file.

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