Fig. 7: Nr6a1 activity is required during mid-to-late gastrulation to induce NCC specification. | Nature Communications

Fig. 7: Nr6a1 activity is required during mid-to-late gastrulation to induce NCC specification.

From: NR6A1 is essential for neural crest cell specification, formation and survival

Fig. 7: Nr6a1 activity is required during mid-to-late gastrulation to induce NCC specification.

In situ hybridization of NCC markers (purple stain) on control (Nr6a1fl/fl) littermates and Nr6a1fl/fl;ERT2-Cre embryos treated with tamoxifen for excision of Nr6a1 at E7.25 and E6.75. Embryos with Nr6a1 excision by E7.25 and littermate controls show normal expression patterns of NCC and EMT markers. Once Nr6a1 is excised by E6.75, NCC and EMT marker expression is lost. Note that some images in this figure were reflected to match the overall orientation of all other images in the figure. A minimum of 3 control and 3 mutant embryos were assayed for each in situ hybridization marker.

Back to article page