Fig. 2: An anatomical profile of ectopic cambia in Japanese wisteria illustrating the collection of cambial cells and their derivatives for transcriptomic analysis.

The inset shows a cross-section of a standard sample of secondary xylem–vascular cambium–secondary phloem taken through tangential cryosectioning (schematic at the bottom left) to produce the transcriptomes. Tissue collection ranged from approximately 40 to 100 sections per sample, with each section ~12 μm thick. This inset is approximately 700 μm in size and represents the average amount of tissue collected per sample (typically 2 mm wide). In the schematic, blue, tan, and pink represent the longitudinal positions of phloem, cambium, and xylem, respectively. Tangential cryosectioning was performed for six samples from each species. Drawings were created in Adobe Illustrator 2025.