Fig. 7: hTOs induce ETP maturation into SP CD4+ and CD8+ T lymphocytes with thymic emigrant phenotypes. | Nature Communications

Fig. 7: hTOs induce ETP maturation into SP CD4+ and CD8+ T lymphocytes with thymic emigrant phenotypes.

From: Combinatory differentiation of human induced pluripotent stem cells generates functional thymic epithelium driving dendritic cell and CD4/CD8 T cell development

Fig. 7: hTOs induce ETP maturation into SP CD4+ and CD8+ T lymphocytes with thymic emigrant phenotypes.

a Flow cytometry of D17, D21, and D35 hTOs showing increasing percentages of CD3+ T cells; representative plots. b Summary of CD3⁺ T cell proportions across independent experiments, with a marked increase observed after 21 days of co-culture. Source data are provided as a Source Data file. c Flow cytometry of D21 hTOs showing αβ and γδ TCR expression within the CD3⁺ fraction (TCRαβ and TCRγδ antibodies). d Flow cytometry of the CD45+CD3+ fraction of D35 hTOs showing SP CD8+ T cells and a less abundant SP CD4+ T cell population. A subset of SP cells displays a CCR7+CD62L+ phenotype, typical of thymic emigrants.

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