Fig. 2: Changes in miRNA profile after spaceflight.
a Distribution of miRNA detection counts based on Tissue and FL, HGC and VGC in LAR and TERM, respectively. A miRNA had to be detected at a count of five in at least 10% of each group to be considered as detected. (Number of biological replicates n: see Supplementary Data 1) Boxplots show the median (center line), the first and third quartiles (box), and whiskers extending to the most extreme data points within 1.5×IQR; values beyond this range are plotted as outliers. Source data are provided as a Source Data file. b Two-dimensional UMAP embedding of the 686 samples colored by tissue of origin. Source data are provided as a Source Data file. c Percentage of variance explained by the variables tissue, extraction method, age, condition, their interactions and the Residual variance as calculated from a PVCA. d Scatterplot of % variance explained by environment versus % variance explained by Spaceflight for each tissue (PVCA). Environment corresponds to Normal Earth conditions (VGC) versus ISS or ISS-matched conditions (HGC and FL). Space effects mean whether the mice were exposed to Spaceflight (FL) or not (HGC and VGC). Source data are provided as a Source Data file. e Counts of the number of deregulated miRNA for each tissue and comparison. A miRNA was deemed to be deregulated if it had an absolute log2 fold-change greater than 1 and an absolute Cohen’s D effect size >0.5 (see Methods). Source data are provided as a Source Data file. f Correlation of fold-changes in FL vs. HGC and FL vs. VGC, primarily capturing the effects of spaceflight, and in HGC vs. VGC and FL vs. VGC, primarily capturing the effects of housing. All correlations shown here were significant (adj. P value < 0.05, Pearson’s correlation, BH adjustment). Source data are provided as a Source Data file.
