Fig. 3: Effects of psilocybin on temperature preference and cold hypersensitivity. | Nature Communications

Fig. 3: Effects of psilocybin on temperature preference and cold hypersensitivity.

From: No evidence of immediate or persistent analgesic effect from a single dose of psilocybin in three mouse models of pain

Fig. 3: Effects of psilocybin on temperature preference and cold hypersensitivity.The alternative text for this image may have been generated using AI.

A–C Thermal Place Preference. Mice are given free access to 30 °C and either 40 C or 20 C surfaces. A CFA-treated, but not CON mice, avoid the 40 °C plate. MOR 10 mg/kg blocks the CFA effect. PSI dose-dependently produces a preference for the 40 °C plate in CFA mice. (CON N = 9 [5 M/4 F]; CFA N = 13 [7 M/6 F]; CFA + MOR N = 9 [5 M/4 F]; PSI 0.3 mg/kg, PSI 2 mg/kg, and PSI 10 mg/kg N = 8 [4 M/4 F]). One-way ANOVA. F(5,49) = 9.147, P = 0.000003. Post-hoc Tukey Test, * P < 0.05 versus CON. Mean ± SEM. B Without CFA treatment, PSI also dose-dependently produces preference for the 40 °C plate. (CON N = 6 [3 M/3 F]; PSI 0.3 mg/kg, PSI 2 mg/kg, and PSI 10 mg/kg N = 8 [4 M/4 F]). One-way ANOVA F(3,26) = 10.34, P = 0.000117. Post-hoc Tukey Test, * P < 0.001 versus CON. Mean ± SEM. C Without CFA treatment, PSI dose-dependently produces aversion for the 20 °C plate. (CON N = 8 [4 M/4 F]; PSI 0.3 mg/kg N = 10 [5 M/5 F]; PSI 2 mg/kg N = 11 [5 M/6 F]; PSI 10 mg/kg N = 8 [4 M/4 F]). One-way ANOVA. F(3,34) = 7.211, P = 0.0007. Post-hoc Tukey Test, *P < 0.001 versus CON. Mean ± SEM. D Formalin treatment + cold plate test (CON, FORM, FORM + PSI 0.3 mg/kg, FORM + PSI 10 mg/kg N = 12 [6 M/6 F]; FORM + PSI 2 mg/kg N = 11 [6 M/5 F] One-way ANOVA. F(5,53) = 8.311, P < 0.000001; Post-hoc Tukey Test *P < 0.05, versus SNI). Mean ± SEM. E–J SNI + cold plate test (N = 12 [6 M/6 F] per group) at acute (7 days after SNI) and chronic phases (21 days after SNI). Acute phase: No dose of PSI had an effect at E 1 hour, F 4 hours, or G 24 hours after injection (One-way ANOVA, 1 hour: F[4,55] = 4.840, P = 0.002, 4 hours: F[4, 55] = 5.571, P = 0.0008, 24 hours F[4,55] = 3.520, P = 0.0125; Post-hoc Tukey Test * P < 0.05, versus SNI). Chronic phase: No dose of PSI had an effect at H 1 hour, I 4 hours, or J 24 hours after injection (One-way ANOVA, 1 hour: F[4,55] = 5.370, P = 0.0010, 4 hours: F[4,55] = 5.571, P = 0.0008, 24 hours: F[4,55] = 6.190, P = 0.0003; Post-hoc Tukey Test *, P < 0.05 versus SNI). Source data are provided as a Source Data file. Mean ± SEM.

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