Fig. 1: AMsh glia respond to both cold and heat stimuli.
From: A Glial Hub-and-Spoke Circuitry in C. elegans orchestrates bidirectional thermosensation
a, Time-lapse heatmap images show GCaMP5.0-based calcium dynamics in ASH neurons and AMsh glia upon cooling. The bath solution was perfused towards the worm, maintaining a temperature of 20 °C, and then cooled from 20 °C to 10 °C within 20 seconds. b, Time-lapse heatmap images show GCaMP5.0-based calcium dynamics in AFD neurons and AMsh glia upon heating. The bath solution was perfused towards the worm, maintaining a temperature of 20 °C, and then heated from 20 °C to 28 °C within 30 seconds. c, Cold-induced calcium responses in AMsh glia in mutants defective in neurotransmitter and neuropeptide release. Left, ΔF/F0 of GCaMP5.0, with solid lines representing the average fluorescence changes and shaded areas indicating standard error of the mean (SEM). Right, Maximum ΔF/F0 of GCaMP5.0, indicating peak calcium responses. Numbers on the columns indicate number of worms tested. d, Heat-induced calcium responses in AMsh glia in mutants defective in neurotransmitter and neuropeptide release. Left, ΔF/F0 of GCaMP5.0. Right, Maximum ΔF/F0 of GCaMP5.0. Numbers on the columns indicate number of worms tested. e-g, Cold and heat-induced calcium responses in isolated cultured AMsh glial cells. e, Time-lapse heatmap images show GCaMP5.0-based calcium dynamics. f, ΔF/F0 and Maximum ΔF/F0 of GCaMP5.0 upon cooling. g, ΔF/F0 and Maximum ΔF/F0 of GCaMP5.0 upon heating in wild type and pde-1,pde-2,pde-3,pde-5 quadruple mutants. Numbers on the columns indicate number of cells tested. Unpaired Mann-Whitney U test, two-tailed. All error bars represent SEM. Unpaired two-tailed t test except Fig.1g. **P < 0.05, and **P < 0.01. Source data are provided as a Source Data file.
