Fig. 5: C1q-Cna binding controls matrix metalloprotease activity and inflammation in skin abscess.
From: Collagen binding adhesin restricts Staphylococcus aureus skin infection
a CFU per gram of homogenized tissue enumerated from either WT C57/BL6 or C1qKO mice, 7 days post infection with WT, Δcna or comp cna bacteria. Representative images of lesions formed at day 7 post infection with lesion sizes measured over the course of the infection for each strain (n = 10 per group, bottom) of WT C57/BL6 (b) or C1qKO (c) mice. d Comprehensive view for the concentrations of inflammatory cytokines measured from abscess tissue 7 days after infection with WT, Δcna or comp cna, in the C1qKO mouse background. Concentrations are presented in logarithmic scale of picogram per mL homogenized tissue. Each column represents results from a single mouse performed in duplicate (n = 5 per group). e Graphical depiction of the known mechanisms by which matrix metalloproteases 9 and 12 can instigate a cascade of neutrophil and macrophage mediated inflammation of skin infection sites that is amplified by the interaction between leukotriene B4 and its receptor (BLT-1). Concentrations of MMP-2, pro-9 and 12 measured from abscess tissue excised from WT BL57 (f) or C1qKO (g) mice (n = 5) infected with WT, Δcna or comp cna bacteria. h Concentrations of leukotriene A-4 hydrolase (LTAH4) measured using an enzyme linked immunosorbent assay, from WT C57/BL6 mice infected with bacterial strains as described in (g) (n = 9 mice per bacterial strain). i Assay similar to (h) measuring the concentrations of leukotriene B4 from homogenized tissue samples (n = 5 mice per group). j Assay similar to (h), quantifying the concentrations of LTAH4 and performed with tissue samples from C1qKO mice infected with bacterial strains as described in (h) (n = 5 mice per group). Results are representative of two independent analyses. Statistical analyses were performed with a two-way ANOVA (b, c) or a one-way ANOVA (a, f–j) with Tukey’s posttest. Data are presented as median values ± SD. *p < 0.05, **p < 0.01 (All adjusted p values are provided in Source Data file). e created in BioRender. Urbina Martinez, M. (2026) https://BioRender.com/byzqrhz.
