Table 3 Strains used in this study
From: Collagen binding adhesin restricts Staphylococcus aureus skin infection
Strain | Description | Reference |
|---|---|---|
MW2 | Cna(+) parent strain | |
MW2 Δcna | MW2 with chromosomal deletion of cna | This study |
MW2 Δcna:pcna | cna introduced on the multicopy plasmid, pCM28 in the MW2 Δcna strain background by electroporation | This study |
USA300 | Cna(−) parent strain | |
USA300:pcna | USA300 with cna introduced on the multicopy plasmid, pCM28 by electroporation | This study |
MW2 hla:tn | hla transposon mutant transduced from Nebraska transposon library into MW2 | This study |
MW2 hla:tnΔcna | hla transposon mutant transduced from Nebraska transposon library into MW2 Δcna | This study |
S. carnosus | TM300 parent strain electroporated with empty vector pCM28 | Dr. Paul Fey, University of Nebraska |
S. carnosus:pcna | TM300 electroporated with pCM28::cna | This study |
E. coli NEB 5α pCM28::cna | E.coli expressing the cna construct with its native promoter, from expression vector pCM28 | This study |
RN4220 | Restriction-defective mutant strain used for cloning | |
E. coli ER2566 pJK11 | E. coli ER2566 electroporated with plasmid expressing pTEV5::Cna174-296 for purification of Cna A-domain | This study |
