Fig. 3: Progeny cells from the SoxC>KikGR and FoxL2>KikGR drivers encompass cnidocyte, secretory, and neural cells. | Nature Communications

Fig. 3: Progeny cells from the SoxC>KikGR and FoxL2>KikGR drivers encompass cnidocyte, secretory, and neural cells.

From: Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis

Fig. 3: Progeny cells from the SoxC>KikGR and FoxL2>KikGR drivers encompass cnidocyte, secretory, and neural cells.

SoxC>KikGR+ (A) and FoxL2>KikGR+ (C) adult polyp body wall at 7 days post reporter photoconversion. Global field of view (5×, left) and high magnification image (40× extended depth of focus (A) or single image (C), right) with photoconverted KikGR signal shown in red and newly expressed KikGR in cyan. SoxC>KikGR+ cells encompass green, orange, and red mature cnidocytes, green, orange, and red neurons (respectively white, yellow, and magenta arrows), and orange secretory cells (yellow filled arrowhead). FoxL2>KikGR+ cells encompass orange (yellow arrow) and red (magenta arrows) neurons among other cells. Scale bars are 500 µm (left), 50 µm (right), and 10 µm in the insets. See Supplementary Figs. 8 and 9 and Supplementary Movies 3–9. Experiment performed at least two times with similar results. Proportion of red, orange and green SoxC>KikGR+ (B) and FoxL2>KikGR+ (D) cell bodies detected at 7 days post-photoconversion. Aboral end body wall images are indicated with a hachure pattern (n = 5, 6, 2, 2, respectively, for oral, SoxC>KikGR; aboral, SoxC>KikGR; and oral and aboral FoxL2>KikGR, average ± SD). E Global scRNA-Seq UMAP projection of 12,352 KikGR+ cells (2764 Elav>KikGR+ cells, 3451 SoxC>KikGR+ cells, and 6137 FoxL2>KikGR+ cells) at 2, 4, and 7 days post-photoconversion, colored by broad cluster identity. F Marker gene expression across clusters. Gene expression is normalized to the highest expression across clusters. Colors correspond to broad cluster annotations, as in (E). Dot size represents the proportion of cells expressing the gene in the cluster. Marker genes names are curated from refs. 13,14,15,16,17,18,39,42,43,47,89 (see Supplementary Data 1). G Cell identity distribution by driver and FACS gating strategy across the SoxC>KikGR, FoxL2>KikGR, and Elav>KikGR drivers. For the FoxL2>KikGR driver, the “KikGR+ no epi.” FACS gate depleted the epithelial epidermis cell fraction. Data is provided as Source Data File 3.

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