Fig. 4: CryoEM structure of MraZ in complex with its cognate DNA promoter region.

a left CryoEM image of the helical “fiber-like” formed by MraZ in complex with a 43 bp dsDNA containing four binding boxes. Middle two opposite orientations of the initial cryoEM 3D volume reconstruction of the fiber-like structure forming DNA concatemers. Right local resolution of the final reconstruction of the complex between four binding DNA boxes with four MraZ subunits (unsharpened map). Scale bar indicated resolution range. b left cryoEM image of the helical “fiber-like” formed by MraZ in complex with a 55 bp dsDNA containing four binding boxes. Middle two opposite orientations of the initial cryoEM 3D volume reconstruction of the fiber-like structure that do not form DNA concatemers. Right local resolution of the final reconstruction of the complex between four binding DNA boxes with four MraZ subunits (unsharpened map). Scale bar indicated resolution range. c Final model fitted in the electron microscopy densities of the complex between DNA-binding boxes with four MraZ subunits (unsharpened map). d Three views of the MraZ electrostatic surface representation in complex with the DNA segment, shown in a cartoon representation. e Structural comparison between the crystal structure of the octamer (brown) and the cryoEM structure of the complex with DNA (different color for each subunit chain) after superposition of one subunit. The DNA segment is shown in a cartoon representation. Distance and angle variation between Ser83 from the DNA segment, shown in a cartoon representation two adjacent DNA binding motifs from two subunits are displayed.