Table 2 Details of the three cryoEM structures of MraZ bound to 43 bp or to 55 bp 4-box dsDNA segment
Fiber 1 43 bp DNA | Fiber 2 55 bp DNA | Octamer-55bp DNA | |
|---|---|---|---|
PDB ID | 9R4J | 9SZ7 | 9SX6 |
EMDB ID | 53569 | 55361 | 55332 |
Data collection | |||
Voltage | 300 kV | 200 kV | 200 kV |
Target defocus | − 0.8,-2.2 | − 1.0,-2.3 | −1.0,-2.3 |
Total exposure [e-/Å2] | 44.48 | 37.4 | 37.4 |
Micrographs | 9195 | 4002 | 4002 |
Particles in the final refinement | 928,675 | 46,906 | 49,625 |
Refinement | |||
Resolution (Å) | 3.36 | 3.87 | 3.57 |
Map sharpening B-factor (Å2) | -141 | -141 | -141 |
Root mean square deviations | |||
Bond lengths (Å) | 0.007 | 0.004 | 0.004 |
Bond angles (°) | 0.87 | 0.74 | 0.66 |
Ramachandran plot | |||
Favored (%) | 89.37 | 80.50 | 92.31 |
Allowed (%) | 10.63 | 17.82 | 7.59 |
Outliers (%) | 0 | 1.64 | 0.09 |
Molprobity | |||
Clash score | 17.18 | 17.02 | 11.19 |
Rotamer outliers (%) | 8.08 | 11.55 | 4.17 |
Overall score | 3.01 | 3.27 | 2.51 |
