Fig. 3: Omission responses are most prominent in superficial layers of the omission response region.
From: The representation of omitted sounds in the mouse auditory cortex
A Mean two-photon fluorescence images displayed as stacked slices at increasing cortical depths. Each image corresponds to a 512 × 512 pixel field of view acquired at the same XY location and shown at its respective depth (50, 100, 200, 300, 400, and 500 µm). Images are displayed in grayscale, with the stack providing a 3D representation of fluorescence across cortical depth. B In the omission-responsive region, the omission response (OR, time-point of area plot in second row indicated by red line, top rows) reduces from more superficial cortical layers to deeper layers (left to right). The response to the standard tone in the omission-responsive region (SR, indicated by the blue line, bottom rows) remained similar in total size as a function of depth. Data shows locally averaged calcium activity from a depth stack from a single mouse (m216). C In the omission-responsive region, the average omission response (red) decreased significantly with depth, with particularly substantial decreases from L1 to L2 (~20%) and from L4 to L5 (~30%). The standard response (SR, blue) was substantially smaller in size, but also showed significant variation with depth, first decreasing from L1 to L2, and then increasing again to L3. Data from depth stacks in 2 mice. OR and SR are both quantified as the average response over show time windows (OR: from 0 ms to 200 ms after omission onset; SR: from 0 ms to 100 ms after standard stimulus onset). D In the primary region, the omission response was substantially smaller (~81%) across depths (p = 0.002, Wilcoxon rank sum test). Shaded areas in C/D show ±1 SEM, where N is corrected by the average correlation of pairs as follows: Neff = N/(1 + (1-N)*C), where C is the average correlation between the activity at different locations117. Layer boundaries drawn in accordance with45. Significance: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) (see Supplementary Data 1 for statistical details). Source data are provided with this paper.
