Fig. 1: B/T injury leads to the early release of cMPCs.
a Experimental design to enrich cMPCs. Whole blood is drawn from mice with burn/tenotomy (B/T) and burn/sham injury controls (B/S), incubated with antibodies targeting leukocytes and magnetic beads; blood is then processed via iChip to enrich cMPCs. Created in BioRender. Karabacak, M. (2026) https://BioRender.com/u7f26d4. b Flow cytometry gating to analyze the marker combination CD45-PDGFRa+CD90+ that contains cMPCs. c CD45-PDGFRa+CD90+ cell numbers following B/T are significantly increased compared to B/S days 1 and 3 post-injury (Day1: n = 4 B/S, n = 9 B/T; Day 3: n = 4 B/S, n = 9 B/T, data are presented as mean values + /-SD). d We repeated the B/T model in transgenic Gli1- and Adipoq-lineage tracing mice and quantified tdTomato cells in enriched blood samples. Left panel shows total number of tdTomato+ cells observed (p = 0.0629), right panel shows %tdTomato labeled cells within PDGFRa/CD90 cMPC gate. e Immunofluorescent histology of uninjured mouse hindlimb and 12 weeks post-injury hindlimb in Gli1CreERT2:ROSA-LSL-TdTomato (shown in purple) with chevrons marking double-positive cells (Col1a1(2.3 kB)-GFP is shown in green, DAPI shown in blue, scale bars = 100 µm, data are presented as mean values ± SEM). f Quantification of the %tdTomato+ cells within Col1a1(2.3)-GFP+ osteoblasts+ (n = 4) and Aggrecan+ chondrocytes (n = 3) in HO anlagen isolated from Gli1creERT2; tdTomato mice (error bars showing mean ± SD). Source data are provided with this paper.
