Fig. 2: PRMT3 regulates adipose ADMA during feeding in an insulin-pAKT-dependent manner.
From: PRMT3-mediated post-translational adaptation to fasting regulates metabolic flexibility
a Western blot analysis of PRMT3, 4 and 5 at d0, d2, d4 and d8 during adipogenic differentiation of preadipocytes isolated from iWAT, representative image from 2 independent repeats. b, c Western blot (b) and quantification (c) of PRMT3, 4 and 5 in eWAT of 12 weeks old male B6N mice at ad libitum, fasted for 36 h, or fasted for 36 h and then refed a normal chow diet for 3–6 h (n = 5, 4 and 4). AL, ad libitum; F-36, fasted 36 h. d, e Western blot analysis (d) and quantification (e) of PRMT3 protein levels in eWAT of 12 weeks old male B6N mice sacrificed at the indicated time points in a 12 h light/dark cycle (n = 4). f, g Western blot analysis (f) and quantification (g) of PRMT3 protein levels in eWAT of 12 weeks old male B6N mice at ad libitum, fasted for 12 h, or fasted for 36 h (n = 4, 5, 5 and 5). AL, ad libitum; F-12, fasted 12 h; F-36, fasted 36 h. (h, i) Western blot analysis (h) and quantification (i) of levels of proteins containing MMA and ADMA in eWAT of 12 weeks old male B6N mice at ad libitum, fasted for 12 h, or fasted for 12 h plus 30 mg/kg SGC707 or fasted for 36 h (n = 5, 6, 7 and 7). AL, ad libitum; F-12, fasted 12 h; F12 + SGC: fasted 12 h plus 30 mg/kg SGC707; F-36, fasted 36 h. Data are mean ± s.e.m. Statistical significance was determined using an unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
