Fig. 7: Transesterification activity of wt-PcAOx and some mutants. | Nature Communications

Fig. 7: Transesterification activity of wt-PcAOx and some mutants.

From: Engineered alcohol oxidases catalyse transesterification in aqueous media without competing hydrolysis

Fig. 7: Transesterification activity of wt-PcAOx and some mutants.The alternative text for this image may have been generated using AI.

a Mutants involving M59A or M59V, b ‘partial’ VPN mutants containing unaltered M59, c PcAOX-VPN compared to its H561A and N601A mutants. Reaction conditions: [PcAOx] = 20 μM, [benzyl alcohol] = 50 mM, [vinyl acetate] = 250 mM, in 50 mM NaPi buffer (pH 7.5) containing 5% v/v DMSO, T = 30°C, 500 rpm, t = 12 h. Experiments were performed as technical duplicates (N = 2), no statistical analysis was performed.

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