Fig. 3: Benchmarking the performance of pMB1 and pSynORI.
From: Engineering plasmids with synthetic origins of replication
A Characterization of growth rate in E. coli cells transformed with pMB1 and pSynORI (measured in units of optical density [OD] at 600 nm). The maximum growth rate is indicated. B Single-cell fluorescence of E. coli cells transformed with pMB1 and pSynORI containing a constitutive RFP expression cassette. The coefficients of variation (CV) of 3 biological replicates of pMB1 are 56.9, 57.8, and 57.9. The CVs of pSynORI are 60.1, 68.2, and 60.0. C Percentage of plasmid multimers in E. coli cells with and without parCBA present. Data show the mass ratio (percentage of total nucleotides in each multimer species) measured by nanopore whole-plasmid sequencing. D Relative copy number of plasmids over time. Relative copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid in E. coli cells (measured in units of fluorescence/optical density [OD]). E The percentage of cells retaining plasmid after culturing is measured by comparing the percentage of cells surviving with and without antibiotic (+AB) selection over a continuous 7-day culture. Cells retaining plasmid are resistant to AB and should grow in both conditions. Data A–D show n = 3 biological replicates. Data in E show 20 colonies collected from n = 3 biological replicates. All error bars and error bands indicate SD. Source data for this figure is available in the Source Data file.
