Fig. 5: A library of compatible pSynORI.
From: Engineering plasmids with synthetic origins of replication
A Matrices showing the relative copy number of pSynORI built with 7 orthogonal pT181 variants. For each variant, combinations with a non-matching target and repressor sRNA (LS) are also included as a control. B A library of 6 pSynORI with different pT181 regulation systems (LS, YS, F6, F4, F4m1, and F3m1) and antibiotic resistances (CmR, SpcR, KanR, AmpR, TetR, and AprR) was created and co-transformed into E. coli cells. The proportions of plasmids in a cell population are measured using nanopore whole-plasmid sequencing. C Plasmid proportion during 8-day continuous culture with antibiotic selection. Cells are re-streaked on day 6, and individual colonies grown from a single cell were picked for day 8 cultures. The samples from days 1, 2, 4, 6, and 8 were processed for sequencing to confirm the presence of plasmids. The ring plot shows the ratio of plasmids on day 1, 6, and 8, with other days shown in Supplementary Fig. 14. Data in A show n = 4 biological replicates and data in C show n = 3 biological replicates. Source data for this figure is available in the Source Data file.
