Fig. 2: Virus community patterns along the groundwater monitoring transect.
From: Diversity and ecological roles of hidden viral players in groundwater microbiomes
a A principal coordinate analysis (PCoA) of Bray-Curtis dissimilarities among the groundwater viruses’ communities was used to assess community structure. This analysis was based on the relative abundance of 257,252 viral operational taxonomic units (vOTUs; ≥5 kb), with each point plotted representing one of the 65 sampled metagenomes, color-coded by the well of origin. PERMANOVA was applied to test for significant differences in viral community composition among wells using Bray–Curtis dissimilarity. (b, c) Macrodiversity (Shannon’s H) and microdiversity (Average π) across the wells. All pairwise comparisons are displayed, with statistically significances (*p < 0.05) indicated by connecting bars, and were conducted using the two-sided Kruskal-Wallis test. d, e Macrodiversity (Shannon’s H), and microdiversity (Average π) in oxic versus anoxic wells. Significant differences between the two groups (**p < 0.01) are indicated by connecting bars. Comparisons were analyzed using the two-sided Mann-Whitney U test. b–e are based on independent biological replicates, where each point represents a single groundwater metagenomic sample collected from an individual well at a specific timepoint. Numbers in parenthesis show the sample sizes: b, c H14 (12 metagenomics samples), H32 (7), H41 (12), H43 (11), H52 (11), H53 (6). d, e Oxic wells (H14, H32, H41, H51): 42 metagenomics samples; Anoxic wells (H43, H52, H53): 23 metagenomics samples. Violin/box plot shows, the center line shows the median; the bounds of the box represent the 25th and 75th percentiles; whiskers extend to 1.5× the interquartile range; points outside this range represent outliers.
