Fig. 1: Localization of VSIG10L in esophageal squamous mucosa.

(Top row) H&E staining of representative human and porcine esophagus FFPE tissue sections, as well as normal esophageal squamous cell line EPC2 grown in 3-dimensional organotypic culture (3D-OTC), showing stratified esophageal squamous mucosa. Images are oriented with luminal side towards the top and basal squamous layer at the bottom. (Bottom row) Custom RNAscope-in situ hybridization (ISH) analyses of respective tissue sections revealing VSIG10L RNA selectively expressed and localized in suprabasal squamous epithelial cell layers (magenta dots), and absent in basal squamous cells. Magnified insets of regions (rectangular boxes) are shown within each of the respective panels, as necessary. All VSIG10L ISH analyses were optimized a priori, and were tested on four human and porcine biologic replicate tissue sections to ascertain reproducibility. Scale bar, 200 µM.