Fig. 1: EI24 is required for homeostasis of AMs.

a Flow cytometric analysis of GFP expression in RPMs, liver macrophages, AMs, and IMs from Ei24-Gfp mice (n = 6 per group). b Ei24 gene expression of AMs sorted from Ei24^fl/fl and Lyz2^creEi24^fl/fl mice (n = 5 per group). c–f The frequencies (left) and absolute numbers (right) of AMs (c), IMs (d), liver macrophages (e), and RPMs (f) in Ei24^fl/fl and Lyz2^creEi24^fl/fl mice (c, n = 8, 13, 7, 9; d, n = 10, 10, 9, 11; e, n = 15, 13, 9, 9; f, n = 16, 16, 7, 7). g Representative flow cytometry of pre-AMs and mature AMs derived from the lungs of Ei24^fl/fland Lyz2^creEi24^fl/fl mice (PND2). h, i The frequencies and absolute numbers of pre-AMs (h) and mature AMs (i) in PND2 Ei24^fl/fl (n = 9) and Lyz2^creEi24^fl/fl (n = 5) mice. j, k Percentages (j) and absolute numbers (k) of mature AMs at the indicated ages in Ei24^fl/fl and Lyz2^creEi24^fl/fl mice (percentages, n = 6, 6, 13, 16, 5, 6; numbers: n = 6, 6, 8, 9, 5, 6). l Frequencies of Ki67⁺ AMs in Lyz2^creEi24^fl/fl and Ei24^fl/fl mice (n = 6 per group). m Frequencies of Annexin V⁺ AMs in adult Ei24^fl/fl and Lyz2^creEi24^fl/fl mice (n = 7 per group). n, o Representative flow cytometry histograms (n) and MFI (o) of Cleaved Caspase-3 expression in AMs from Lyz2^creEi24^fl/fl (n = 6) and Ei24^fl/fl (n = 5) mice. p, q MFI of Cleaved Caspase-3 (p) in AMs and absolute AM numbers (q) in Lyz2^creEi24^fl/fl mice treated with vehicle- (n = 7) or Z-VAD-FMK (n = 6). Data are pooled from (a–f, h–q) two to three independent experiments and presented as the mean ± SEM. Data were analyzed by one-way ANOVA followed by a Tukey’s test (a), two-tailed unpaired t-test (b–f, h, i, l, m, o–q), and two-way analysis of variance (ANOVA) with Šidák’s multiple comparisons test (j, k). Source data are provided as a Source Data file.