Fig. 3: Trem2 knockout inhibits PTX-promoted lung metastasis in breast cancer in mice. | Nature Communications

Fig. 3: Trem2 knockout inhibits PTX-promoted lung metastasis in breast cancer in mice.

From: Paclitaxel drives TREM2+ macrophage expansion underlying its inferior therapeutic efficacy compared to Nab-paclitaxel

Fig. 3: Trem2 knockout inhibits PTX-promoted lung metastasis in breast cancer in mice.

a Schematic of the experimental design. Trem2+/+ and Trem2−/− mice were orthotopically injected with Py8119 cells and treated with DMSO or PTX, respectively. Created in BioRender. Xing, Y. (2026) https://BioRender.com/nsp747pb Photographs of representative tumors from mice that received the indicated treatments on day 14. c Tumor growth curves (left) and tumor weights (right) of Py8119 tumors in mice that received the indicated treatments (n = 6 mice per group). d Representative bright-field images (left) of lungs from Py8119 cell-bearing mice that received the indicated treatments. Quantification of the total number of lung-surface metastases is shown (right) (n = 6 mice per group). e Representative H&E-stained images of lungs from mice. f Representative flow cytometric analysis of histograms showing the proportion of TREM2-expressing macrophages in tumors. g Quantification of flow cytometric analysis showing the proportion of TREM2-expressing macrophages in tumors (n = 3 mice per group). h Representative flow cytometric analysis of pseudo-color plots showing the proportion of TREM2-expressing macrophages in the PB of mice (n = 3 mice per group). i Schematic of the experimental design. Trem2-/- mice were co-injected orthotopically with Py8119 cells and BMDMs. Created in BioRender. Xing, Y. (2026) https://BioRender.com/nsp747pj Photographs of representative tumors from Trem2-/- mice that received the indicated treatments on day 17. k Tumor growth curves (left) and tumor weights (right) of Py8119 tumors in Trem2-/- mice (n = 6 mice per group). l Representative bright-field images (left) of lungs from Trem2-/- mice that received the indicated inoculations. Quantification of the total number of lung-surface metastases is shown (right) (n = 6 mice per group). Data are shown as means ± S.D. and were analyzed by two-sided unpaired Student’s t test (g), two-sided one-way ANOVA followed by Tukey’s test (c, d, k and l) and two-sided two-way ANOVA followed by Tukey’s test (c and k). Source data are provided as a Source Data file.

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