Fig. 3: Cell-specific delivery of Cre recombinase mediated by CBB lipidoids in the mT/mG mouse retina. | Nature Communications

Fig. 3: Cell-specific delivery of Cre recombinase mediated by CBB lipidoids in the mT/mG mouse retina.

From: A combinatorial synthetic strategy for developing genome-editing protein-delivery agents targeting mouse retina

Fig. 3: Cell-specific delivery of Cre recombinase mediated by CBB lipidoids in the mT/mG mouse retina.

a Schematic representation of the genetic construct of the mT/mG Cre-reporter-mouse model. The delivery of Cre recombinase to the nucleus triggers excision of the floxed tdTomato and STOP-polyA casette, enabling the expression of eGFP. b Representative images indicating the switch in expression from tdTomato to eGFP, one week after subretinal injection of Cre recombinase complexed with CBB lipidoids. The RPE layer is orientated toward the top. C, Cre recombinase only. The scale is provided in micrometers. Images are representative of at least 3 eyes.

Back to article page