Fig. 3: DNA scaffold reorganization in PIC.
From: Structural basis of pausing during transcription initiation in mycobacterium tuberculosis
A,B The RNA/DNA hybrid scaffold is visualized with its corresponding cryo-EM density map (transparent gray mesh), highlighting structural integration within the transcriptional complex. C,D σ4 domain engagement with promoter −35 element. IC6 (C) and PIC7 (D) structures shown in dual orientations (front and side views), which also show different modes of combination. E,F Functional validation of interaction motifs. EMSA quantifies DNA binding affinity changes caused by σ4 point mutations and their effects on complex formation using the DNA substrate shown in Supplementary Fig. 1H (E). The Mango-fluorescence transcription assay reveals σ4-dependent transcriptional activation (F). The data are presented as mean ± S.E.M. n = 3 independent experiments. Source data are provided as a Source Data file.
