Fig. 1: Enzyme activity and cryo-EM structures of Fks1 and Fks2.
From: Inhibition mechanism of the fungal β−1,3-glucan synthases by triterpenoid antifungal drugs
a Cartoon schematic of β-1,3-glucan synthase. b In vitro UDP-Glo glycosyltransferase assay with purified Fks1 and Fks2 with Rho1. The reaction without FKS was used as the control. Data points represent the mean ± SD (n = 3). c Cryo-EM map and atomic model of endogenous Fks1. d Cryo-EM map of endogenous Fks2. e Structural comparison between Fks1-apo (green) and Fks2-apo (blue). f Topological model of Fks1 and Fks2. g Close-up view of the region around HH1A-1B. h Close-up view of the highlighted box region in panel e. The extracellular loop between TM1 and TM2 directly interacts with the extracellular loop between TM7 and TM8 at the outer side of the plasma membrane. Source data are provided as a Source Data file.
