Fig. 5: Structure of Fks1 in an open state.
From: Inhibition mechanism of the fungal β−1,3-glucan synthases by triterpenoid antifungal drugs
a Structural comparison between Fks1–open (color) and Fks1–apo (gray). The TMD2 shifts in the membrane by ~5 Å. b A close-up view of the channel entry region in Fks1–open viewed from the cytosol. c Superimposed channel entry regions of Fks1–EFU and Fks1–apo in the same view as panel (b). d Growth complementation of fks1Δ cells with empty plasmid (fks1Δ), or plasmid carrying either WT Fks1 (FKS1) or mutants. Cells were serially diluted, spotted onto synthetic Histidine-dropout medium plates (SD-His) and SD-His plates with 0.1 μg/mL FK506, and incubated at 30 °C for 2 days. e In vitro UDP-Glo glycosyltransferase assay of wild-type Fks1 and Fks1 mutants. The reaction without FKS was used as the control. Data points represent the mean ± SD (n = 3). Source data are provided as a Source Data file.
