Fig. 2: Antibody profiles of mice immunized intramuscularly with cnsLTP1-mRNA, Pru p 3-mRNA, Par j 2-mRNA, or cnsLTP1-protein.
a Injection and bleeding schedule for immunized mice. b Total antibody titers were measured by ELISA on day 63 for selected isotypes in mice immunised with PBS (black dots), cnsLTP1-protein (pink squares), cnsLTP1-mRNA (teal triangles), Pru p 3-mRNA (orange upward triangles) or Par j 2-mRNA (green rhombus) c cnsLTP1 allergen-specific IgG1 antibody responses measured by ELISA following injection with no doses (day 0), 1 dose (day 21), 2 doses (day 42), or 3 doses (day 63) of PBS (black dots), cnsLTP1-protein (pink squares), cnsLTP1-mRNA (teal triangles), Pru p 3-mRNA (orange upward triangles) or Par j 2-mRNA (green rhombus) d cnsLTP1 allergen-specific IgG2a antibody responses measured by ELISA following injection with no doses (day 0), 1 dose (day 21), 2 doses (day 42), or 3 doses (day 63) PBS (black dots), cnsLTP1-protein (pink squares), cnsLTP1-mRNA (teal triangles), Pru p 3-mRNA (orange upward triangles) or Par j 2-mRNA (green rhombus). Bar heights are means ± SD. Statistical significance was determined using a two-way ANOVA with Dunnett’s multiple comparisons test comparing each sample in a group to the respective PBS control: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Figures were created in BioRender. Rivera de Torre, E. (2026) https://BioRender.com/8olw5jx, and graphs were plotted using GraphPad Prism version 10.
