Fig. 5: Mouse serum–mediated inhibition of human IgE binding to nsLTP allergens assessed by ELISA.

a Recognition of recombinant nsLTP allergens by human serum IgE from selected patients. The experiment was performed twice with technical triplicates. c Inhibition of human serum IgE binding to nsLTP allergens by mouse serum antibodies measured by ELISA after immunization with cnsLTP1 in mRNA (teal) and protein (pink) formulations. Mouse serum was added in increasing dilutions (1:10 to 1:10,000), while human serum was used at a 1:10 dilution, as described in the Methods. Dotted lines indicate non-linear regression fits used to calculate IC₅₀ values. Data were normalized to the maximum signal of technical triplicates. c IC₅₀ values calculated from the inhibition curves shown in (b) for immunizations with cnsLTP1 in mRNA (teal) and protein (pink) formulations. Data are presented as mean ± SD. Individual IC₅₀ values are reported in Supplementary Table 3. Statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparisons test, comparing each sample to the respective PBS control (*P ≤ 0.05). Graphs were plotted using GraphPad Prism version 10.