Fig. 7: Evaluation of the allergic anaphylactic mouse model immunized intramuscularly with cnsLTP1 mRNA-LNP.
a Schematic overview of the sensitization, treatment, and allergen challenge protocol, including timing of intramuscular immunization, blood collection, intraperitoneal challenge, and body temperature monitoring. b Change in core body temperature following intraperitoneal challenge with Pru p 3.in sensitized anaphylactic control (black), and sensitized mice treated with cnsLTP1 mRNA-LNP (teal) mice. Statistical significance was determined using the two-sided Mann–Whitney test. c Serum mouse mast cell protease-1 (mMCP-1) concentrations measured by ELISA in non-sensitized healthy control (grey), sensitized anaphylactic control (black), and sensitized mice treated with cnsLTP1 mRNA-LNP (teal) mice. d Allergen-specific IgG1 antibody levels measured by ELISA in non-sensitized healthy control (grey), sensitized anaphylactic control (black), and sensitized mice treated with cnsLTP1 mRNA-LNP (teal) mice. e Allergen-specific IgG2a antibody levels measured by ELISA in non-sensitized healthy control (grey), sensitized anaphylactic control (black), and sensitized mice treated with cnsLTP1 mRNA-LNP (teal) mice. f Allergen-specific IgG2b antibody levels measured by ELISA non-sensitized healthy control (grey), sensitized anaphylactic control (black), and sensitized mice treated with cnsLTP1 mRNA-LNP (teal) mice. g Pru p 3–specific IgE levels determined by ELISA in non-sensitized healthy control (grey), sensitized anaphylactic control (black), and sensitized mice treated with cnsLTP1 mRNA-LNP (teal) mice. All data are presented as mean ± standard SD, with eight mice per group. Statistical significance was determined using one-way ANOVA with two-sided Dunnett’s multiple comparisons test (unless otherwise stated), comparing each group to the anaphylactic control: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Exact P values are indicated where P > 0.05. Schematics were created in BioRender. Rivera de torre, E. (2026) https://BioRender.com/q5fxgfj, and graphs were plotted using GraphPad Prism version 10.
