Fig. 1: Protein purification and functional characterization.
From: Molecular mechanism of phosphate import by the bacterial PstSCAB transporter
a Purification of PstCAB. Size exclusion chromatography (SEC) trace and SDS-PAGE gel image were shown. SEC was repeated twice with similar results. b ATPase activity of wild-type PstCAB and PstCABE179Q. c Concentration-dependent ATPase activity of wild-type PstCAB. Data were fit with a model of substrate inhibition. d, e Effect of Pi (d) and PstS (e) on ATPase activity of wild-type PstCAB. f Binding curves for Pi with purified PstCAB, measured with MST assays. In b–f data represent mean ± SD (biological replicates, n = 3), except for the control in (f) (biological replicates, n = 2). Source data are provided as a Source data file.
