Fig. 6: Loss of hnRNPM results in aberrant pre-mRNA splicing in oocytes.
From: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development
a The numbers and categories of annotated differential alternative splicing (AS) events detected in GV oocytes following Hnrnpm depletion. b GO term analysis of differentially alternative splicing (AS) genes by Metascape. c Diagrammatic representation of alternative splicing quantification methodology using percentage spliced in (PSI) values. The PSI (Percent Spliced In) represents the relative abundance of transcripts containing a specific exon or splice site. d, e Sashimi plots illustrating differential alternative splicing events of cell lattice formation-associated genes (Khdc3 and Nlrp14) and meiotic maturation-related genes (Cenph and Zar1l). PCR validation of these candidate genes confirmed the splicing alterations in Hnrnpm cKO oocytes. Schematic of alternatively spliced exons. Bar plots display the percentage spliced in (PSI) values of control and Hnrnpm cKO oocytes, calculated as PSI = splice_in/(splice_in + splice_out). Two-sided Student’s t-tests. Data are presented as mean ± SEM. n = 3. p = 0.0022 (Khdc3), 0.0012 (Nlrp14), 0.0001 (Cenph), 0.0002 (Zar1l). Representative results shown in (d, e) were obtained from at least three independent experiments with similar results. Source data (b, d, e) are provided as a Source data file.
