Fig. 5: Effects of cnd1-K658E mutation on condensin distribution across the genome.

a Genome-wide distributions of condensin were determined by ChIP-seq using mitotic cells expressing wild-type Cnd1 (Cnd1-WT-Myc) or Cnd1-K658E mutant (Cnd1-K658E-Myc) from the endogenous cnd1 locus (top two rows). Mitotic cells were prepared in YEA medium using the cdc25-22 G2/M block-release method and harvested 40 min after release. In addition, the genome-wide profiles of Cnd1-Pk in mitotic cells expressing wild-type Pmc4 from a plasmid (Pmc4 WT) or lacking exogenous Pmc4 expression (Pmc4 depletion) are shown (bottom two rows). Endogenous Pmc4 was depleted by the AID system. Mitotic cells were prepared in EMM media by the cdc25-22 G2/M block-release procedure and harvested 60 min after release. Condensin enrichment across a 130-kb genomic region on chromosome I (b), the Ace2-regulated eng1 gene locus (c), and the highly transcribed rps23 gene locus (d). Average condensin enrichment at Ace2 target and highly transcribed Pol II genes (e) and at tRNA and 5S rRNA Pol III genes (f) in the indicated samples. Highly transcribed Pol II genes were classified as described in Fig. 1e, and genes bound by both Cnd1 and Cut14 (defined in Fig. 1f) were designated as condensin-associated genes. Genes overlapping between these two categories were annotated as the highly transcribed, condensin-binding genes. g ChIP-qPCR analysis verifying enrichment of Cnd1-WT-Myc and Cnd1-K658E at the eng1, rps23, and tRNA-ser loci in mitotic cells. Experiments were performed in triplicate as described in (a). h ChIP-qPCR analysis verifying the enrichment of Cnd1-Pk at the eng1, rps23, and tRNA-ser loci in mitotic cells expressing wild-type Pmc4 from a plasmid (Pmc4 WT) or lacking exogenous Pmc4 expression (Pmc4 depletion). Experiments were performed in triplicate as described in (a). Data in (g, h) are represented as mean ± SD. Source data are provided as a Source Data file.