Fig. 5: DFRSox2 enhances chromatin invasion through nonspecific interactions with DNA. | Nature Communications

Fig. 5: DFRSox2 enhances chromatin invasion through nonspecific interactions with DNA.

From: Electrostatic properties of disordered regions control transcription factor search and pioneer activity

Fig. 5: DFRSox2 enhances chromatin invasion through nonspecific interactions with DNA.

a Scheme of single-molecule experiment to detect Sox TF binding to chromatin fibers. b Fluorescence time-traces of Sox TFs interacting with CF+ and CF−. c Specific on-rate of Sox TFs to mononucleosomes and chromatin. The background on-rates (gray) measured with control constructs (MN−, CF−) are overlaid with corresponding constructs containing the target site (green). Symbol: replicate (N = 4 for Sox2 with MN + 2 and CF+, and Sox2a with MN + 2; N = 3 for all the other cases). Bar: mean. Cap: SD. d On-rate of Sox TFs for different nucleosomes. Symbol: replicate (N = 4 for Sox2 with MN + 2 and CF+, and Sox2a with MN + 2; N = 3 for all the other cases). Bar: mean. Cap: SD. Statistical significance was assessed using two-tailed t-tests (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).

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