Fig. 6: DFR_Sox2 enhances pioneering activity.

a Cell sorting strategy for ChIP-seq and ATAC-seq: mESCs expressing Halo-Sox2 or -Sox2a were treated with SiR647-Halo ligand and FACS-sorted using indicated gating windows (black) corresponding to near-identical TF expression levels (see also Supplementary Table 13). b Genomic regions enriched by Sox2 (blue) and Sox2a (pink). Two replicates for each Sox TF were merged. c Heatmaps of ChIP-seq data (RPKM) across Sox-enriched regions. d Genome tracks of genomic regions displaying ChIP-seq and ATAC-seq (RPKM) signals, representing Sox2/Sox2a-enriched CORs, and ±10% subregions. The values indicate the number of peaks in each region. e Heatmaps of ChIP-seq and ATAC-seq data (RPKM) aligned to the log2-fold change in ChIP scores of Sox2 over Sox2a across CORs (2/2a) and ±10% subregions. 2/C: log2-fold change in ATAC scores of Sox2 over Ctrl; 2a/C: log2-fold change in ATAC scores of Sox2a over Ctrl. Log2-fold changes were calculated based on the peak signal in bed files. f Mean score profile of Ctrl ATAC data for Sox2-enriched and Sox2a-enriched CORs. g Mean score profile of log2-fold change in ATAC scores of Sox TFs over Ctrl across CORs. h Mean score profile of Ctrl ATAC data for mESCs overexpressing Sox TFs across ±10% subregions. i Mean score profile of the log2-fold change in ATAC scores of mESCs overexpressing Sox TFs compared to Ctrl mESCs across ±10% subregions.