Fig. 2: RANMP for ER-SRS.
From: Fluorescence-free single-molecule microscopy via electronic resonance stimulated Raman scattering
A Molecular structures of the RANMP series in which four nitrile groups conjugated to the highly conjugated structures with near-infrared absorption and fluorescence quantum yield below the detection limit of our instrumentation: RANMP, RANMP-4F, RANMP-4Cl, and RANMP-T. B Sample geometry of chromophore film for SRS detection in transillumination geometry. C Time trace of ER-SRS signal of RANMP-T. Blue curve is Gaussian fitting to the trace when the Stokes pulse precedes the pump. Red line is exponential fitting to the trace when pump pulse precedes Stokes. D Pump power dependence of ER-SRS signal. E ER-SRS spectra of the four RANMPs at the optimal combination of pump and Stokes wavelengths. Lines indicate spline interpolation. Error bars represent the mean ± standard deviation (n = 5), with individual replicates shown as purple dots. F Summary of ER-SRS spectra at the optimal condition in (E) in terms of the absorption max (λmax), wavelength difference of the pump wavelength from the absorption max (λmax − λpump), S/B ratio and signal enhancement in comparison to the EPR-SRS signal of Rho800 with fixed Stokes wavelength at 1031.2 nm as in previous studies. Intensities are reported in arbitrary units (a.u.).
