Fig. 8: Comparison of HCV-1 E2mc3 and sE1E2.Cut1+2.SPYΔN vaccine-induced neutralizing antibody responses. | Nature Communications

Fig. 8: Comparison of HCV-1 E2mc3 and sE1E2.Cut1+2.SPYΔN vaccine-induced neutralizing antibody responses.

From: Native-like soluble E1E2 glycoprotein heterodimers on self-assembling protein nanoparticles for hepatitis C virus vaccine design

Fig. 8: Comparison of HCV-1 E2mc3 and sE1E2.Cut1+2.SPYΔN vaccine-induced neutralizing antibody responses.

a Surface model of the HCV-1 E2mc3-10GS-FR SApNP (left) and representative nsEM micrograph of ExpiCHO-expressed, AR3C/SEC-purified E2mc3-10GS-FR SApNPs (right). The FR scaffold is shown in gray, and E2mc3 in green. b ELISA-derived EC50 (µg/ml) values for HCV-1 E2mc3 and E2mc3-10GS-FR binding to four NAbs and CD81-Fc. Antigens were produced in ExpiCHO cells and purified using an AR3C antibody column and SEC. When OD450 < 0.5 at 10 µg/ml, binding was considered negligible, and EC50 values were set to 10 µg/ml. c NAb responses induced by HCV-1 E2mc3, E2mc3-10GS-FR, sE1E2.Cut1+2.SPYΔN-His6, sE1E2.Cut1+2.SPYΔN-10GS-FR, and sE1E2.Ext1.Cut2.SPYΔN-His6 vaccines against H77 HCVpp at week 11. ID50 titers were calculated from HCVpp neutralization assays (0–100% constraint). Data points are shown as geometric mean ± geometric SD. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test, or two-tailed unpaired t-tests for two-group comparisons. Statistical significance is indicated as ns (not significant).

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