Fig. 9: TAX1BP1 knockout leads to the accumulation of fragmented Golgi upon STING activation.
From: Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy
A Transmission electron microscopy showing ultrastructure of Golgi in control (LysM-Cre) BMDMs indicating intact Golgi stacks and cisternae. G- Golgi, M- Mitochondria, N-Nucleus. B Ultrastructure of Golgi in control BMDMs treated with diABZI for 4 h reveals swollen and fragmented Golgi with phagophores. Black arrow-swollen and fragmented Golgi, double-headed arrow- phagophores, black arrowhead- autolysosomes. C Control BMDMs treated with diABZI for 4 h. Black arrow-swollen and fragmented Golgi, double-headed arrow-phagophores, black arrowhead- autolysosomes. D Ultrastructure of Golgi in control BMDMs treated with diABZI for 4 h reveals autolysosomes with fragmented Golgi undergoing degradation. Black arrow-swollen and fragmented Golgi, black arrowhead- autolysosomes. E Ultrastructure of Golgi in Tax1bp1–/– (Tax1bp1fl/flLysM-Cre) BMDMs showing Golgi stacks and cisternae. G- Golgi, M- Mitochondria, N-Nucleus. F Tax1bp1–/– (Tax1bp1fl/flLysM-Cre) BMDMs treated with diABZI for 4 h reveals swollen and fragmented Golgi. Black arrow-swollen and fragmented Golgi, yellow arrowhead- dark patches of protein aggregates. G Ultrastructure of Golgi in Tax1bp1–/– (Tax1bp1fl/flLysM-Cre) BMDMs treated with diABZI for 4 h. Black arrow-swollen and fragmented Golgi, yellow arrowhead- dark patches of protein aggregates. H Ultrastructure of Golgi in Tax1bp1–/– BMDMs treated with diABZI for 4 h. Black arrow-swollen and fragmented Golgi. I Quantification of autophagic vacuoles/vesicles (AV) per cell. P values from left to right: P = 0.8734, P < 0.0001, P < 0.0001, P = 0.0023. The results are expressed as the mean ± SD (n = 10 micrographs per condition). ****P < 0.0001; **P < 0.01; *P < 0.05; ns = significant. Two-way ANOVA with Sidak’s multiple comparisons test was used. J Immunoblotting with the indicated antibodies using lysates from control and TAX1BP1-KO THP-1 cells treated with diABZI and/or C53 for 6 h. The experiment is representative of three independent experiments with similar results. Source data are provided as a Source data file.
