Fig. 7: Disruption of one single neuron induces transient failure of eyeblink and ensemble response to visual stimuli and fast recovery.
a The eyelid closure amplitude of mice in response to the first CS after photostimulation (P.S.) and photodisruption (P.D.) respectively. CS: before photostimulation or photodisruption. Gray: the threshold of eyelid closure set as 8% of the full amplitude. n = 34, 17 trials from 6 mice respectively for CS and photostimulation, n = 17 trials from 5 mice for photodisruption. p = 6.4 × 10-7, n.s., no significant difference. Two-tailed Mann-Whitney test. b The quantified eyelid closure (CR, conditional response) to the first visual stimulus after photostimulation or photodisruption. c Upper: the eyelid closure amplitude along each CS test after photodisruption. Gray: the threshold of eyelid closure set as 8% of the full amplitude. Bottom: the percentage of recovered ensembles that responded eyeblink to visual stimuli after photodisruption i.e., n = 17 disruptions from 5 mice. CR: conditional responses. d The eyelid closure response after photodisruption was tested by CS after resting for different CS times or different durations. Gray: the threshold of eyelid closure set as 8% of the full amplitude. n = 34 CS trials from 5 mice. e The neurons responded to the flicker of the square (3, E). Yellow: on neurons; Red: off neurons; Blue: neurons respond to both on and off. Scale bar: 50 μm. n = 6 disruptions from 3 mice. f The correlation of the neurons in (e) indicated the ‘On’ ensemble (neurons 1-10) and ‘Off’ ensemble (neurons 13–19). * p < 0.0001; Pearson correlation coefficient, two-tailed t test. g The Ca2+ activities of neuron 1–19 before and after photodisruption of neuron 1. n = 6 disruptions from 3 mice. h The amplitude of the neurons responded to the first visual stimulus after the photodisruption of the ensemble neurons. n = 18, 14 neurons respectively for on-cell and off-cell from 3 mice. p = 0.04 (on-cell), 0.0245 (off-cell); Two-tailed Wilcoxon matched-pairs signed rank test. i The extent of eyelid closure triggered by stimulating adjacent ensemble neurons right after photodisrupting a single individual ensemble neuron (p = 0.001; n = 7 photostimulation trials from 4 neurons, 2 mice; Two-tailed Mann-Whitney test). Gray: the threshold of eyelid closure set as 8% of the full amplitude. j The normalized Ca2+ amplitude of all neurons along with repeated visual stimuli. Red background: the first five CS after photodisruption. p < 0.04; n = 17 neurons, Two-tailed Mann-Whitney test. k Correlation of ensemble neuronal responses to the first (left) and 10th (right) CS after photodisruption, respectively (* p < 0.0001; Pearson correlation coefficient, two-tailed t test). Error bars for the averaged plot indicate mean ± s.e.m. Source data are provided as a Source Data file.
