Fig. 1: Pulmonary epithelial-specific BDNF is negatively correlated with the progression of ALI. | Nature Communications

Fig. 1: Pulmonary epithelial-specific BDNF is negatively correlated with the progression of ALI.

From: Brain-derived neurotrophic factor and the derived dodecapeptide function as Toll-like receptor 4 antagonists in acute lung injury

Fig. 1: Pulmonary epithelial-specific BDNF is negatively correlated with the progression of ALI.The alternative text for this image may have been generated using AI.

A Heatmap of typical NTKs (including Artn, Bdnf, Cntf, Gdnf, Ngf, Nrtn, Ntf3, Ntf5, and Pspn) from both self-performed sequencing data (PRJNA1183441 in SRA database) and publicly available datasets (GSE233206 and GSE214249 in the GEO database). B Veen diagram of differentially expressed genes in panel A. C The count value of Bdnf in GSE204709 across various infection time points (0–14 d). D The FPKM value of Bdnf in GSE199546 across various infection time points (0–60 h). E BDNF content in serum and BALF of non-ARDS and ARDS patients. F BDNF content in serum and BALF of mice treated with or without LPS. G tSNE plot of 13 cell clusters in mouse lungs. H Dot plot showing the expression of marker genes for the 13 cell clusters. I Feature plots illustrating the expression scores of Bdnf in combined and separate Sham and CLP groups. J Dot plot showing the expression of Bdnf in 13 cell clusters of the Sham and CLP group. K The protein level of BDNF in lung tissues from mice treated with or without LPS, and GAPDH was used as loading control. Corresponding relative BALF IL-6 levels for each group are shown on the right. L Protein level of BDNF in primary epithelial cells (ECs), primary fibroblasts (PFs), and primary macrophages (PMs) with or without 500 ng/mL LPS treatment for 24 h. Data are represented as mean ± SEM by unpaired 2-tailed Student’s t-test (E, F, K). Source data are provided as a Source Data file.

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