Fig. 5: The anti-tumor immunity in Tmem175^−/− mice is inhibited by anti-IL-1β and anti-IL-18.

a–l Tmem175^−/− and WT mice (n = 5 mice) were treated with 100 µg/100 µL anti-IL-1β or Armenian hamster IgG isotype intraperitoneally 1 day before the subcutaneous implantation with \(1\times {10}^{6}\) B16-F10. Then same doses of IgG were injected intraperitoneally on day 6 and 13. Mice were euthanized on day 14 to weigh the tumors and analyze the TME through FCM. b The anti-tumor effects of TMEM175 knockout were suppressed by anti-IL-1β. c Expression of MHC-II in TAMs (gated from CD45⁺ CD11b⁺ F4/80⁺ cells) was significantly attenuated by anti-IL-1β in Tmem175^−/− mice. d Expression of CD206 in TAMs (gated from CD45⁺ CD11b⁺ F4/80⁺ cells) was not obviously altered by anti-IL-1β in Tmem175^−/− mice. e–h Recruitment, activation, IFN-γ expression, and granzyme B expression in CD4⁺ T cells (gated from CD3⁺ cells) were significantly diminished by anti-IL-1β in Tmem175^−/− mice. i–l Recruitment, activation, IFN-γ expression, and granzyme B expression in CD8⁺ T cells (gated from CD3⁺ cells) were significantly diminished by anti-IL-1β in Tmem175^−/− mice. m–x Tmem175^−/− and WT mice (n = 5 mice) were treated with 100 µg/100 µL anti-IL-18 or rat IgG2a isotype intraperitoneally 1 day before the subcutaneous implantation with \(1\times {10}^{6}\) B16-F10. Then same doses of IgG were injected intraperitoneally on day 6 and 13. Mice were euthanized on day 14 to weigh the tumors and analyze the TME through FCM. n The anti-tumor effects of TMEM175 knockout were suppressed by anti-IL-18. o Expression of MHC-II in TAMs (gated from CD45⁺ CD11b⁺ F4/80⁺ cells) was significantly attenuated by anti-IL-18 in Tmem175^−/− mice. p Expression of CD206 in TAMs (gated from CD45⁺ CD11b⁺ F4/80⁺ cells) was not obviously altered by anti-IL-18 in Tmem175^−/− mice. q–t Recruitment, activation, IFN-γ expression, and granzyme B expression in CD4⁺ T cells (gated from CD3⁺ cells) were significantly diminished by anti-IL-18 in Tmem175^−/− mice. Activated CD4⁺ T cells (gated from CD3⁺ cells) were significantly diminished by anti-IL-18 in WT mice. u–x Recruitment, activation, IFN-γ expression, and granzyme B expression in CD8⁺ T cells (gated from CD3⁺ cells) were significantly diminished by anti-IL-18 in Tmem175^−/− mice. Recruitment, activation, and granzyme B expression in CD8⁺ T cells (gated from CD3⁺ cells) were significantly diminished by anti-IL-18 in WT mice. Representative results from two independent experiments are presented as mean ± SEM, ns denotes not significant. Statistical significances in (b–l and n–x) were determined by two-way ANOVA followed by Holm-Sidak’s multiple comparisons test. Source data are provided as a Source data file. a, m Created in BioRender. Wang, Y. (2026) https://BioRender.com/yaf354h.