Fig. 1: Two high-affinity nanobodies bind non-overlapping epitopes. | Nature Communications

Fig. 1: Two high-affinity nanobodies bind non-overlapping epitopes.

From: Nanobody-based IgG simultaneously inhibit the allergenic and enzymatic activity of the dominant honeybee venom allergen

Fig. 1: Two high-affinity nanobodies bind non-overlapping epitopes.The alt text for this image may have been generated using AI.

a Purified nanobodies were assessed by SDS-PAGE. b, c Binding kinetics of the nanobodies (Nb) were obtained with BLI using biosensors loaded with nApi m 1. b Kinetic constants were calculated using a 1:1 binding model (black lines). c Simultaneously binding was assessed by binding of AM1-1 (green) followed by binding of AM1-4 (blue) (left) or vice versa (right).

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