Fig. 7: Bispecific nb-hIgG1 reduces mast cell activation and anaphylaxis in vivo.
a Schematic representation of the systemic anaphylaxis mouse model. Non-sensitized mice (n = 12) and mice sensitized by three weekly intraperitoneal (IP) injections of Honeybee venom (HBV) extract were challenged with Api m 1 at day 28. Sensitized mice received treatment with either PBS (n = 10) or bispecific nb-hIgG1 (n = 12) four hours prior to the challenge. b Blood was collected after the challenge and serum MCPT-1 concentration was measured by ELISA. Data are presented as mean ± SD and each symbol represents a single mouse. Statistical analysis was performed using one-way ANOVA followed by Tukey multiple comparison test (****p < 0.0001; ns: non-significant). c Body temperature was measured every 15 min for 90 min after the challenge. Data are presented as mean ± SD. d The area under the curve (AUC) of the temperature curves were calculated and statistical analysis was performed using one-way ANOVA followed by Tukey multiple comparison test (****p < 0.0001; ns:non-significant). Data are presented as mean ± SD and each symbol represents a single mouse. Source data are provided as a Source Data file.
