Fig. 1: Characterization of the genome-wide distribution of H3K4me1 across diverse species.

a Heatmaps showing the individual gene distribution of H3K4me1, together with their associated transcription level (log₂-transformed). All genes are sorted based on enrichment of H3K4me1. Gradient colors indicate levels of H3K4me1 enrichment and transcription, respectively. b Integrative genomic distribution of H3K4me1. c Scatter plots showing the correlation between H3K4me1 level and gene transcription level. The species (from left to right) are Saccharomyces cerevisiae, Drosophila melanogaster, Mus musculus, Homo sapiens, Chlamydomonas reinhardtii, Physcomitrium patens, Arabidopsis thaliana, and Oryza sativa, respectively. The transcribed genes (RPKM > 1) enriched with the H3K4me1 modification were divided into 100 groups based on the levels of their transcription and modification. The average values of each group of modifications and transcription were calculated for analysis. The heatmaps and plots present the region from 3 kb upstream of the transcription start site (TSS) to 3 kb downstream of the transcription termination site (TTS). For scatter plots, Spearman’s rank correlation coefficient (rho) indicates the correlation between the methylation level and gene transcription level. The p-values were determined based on two-sided Spearman’s rank correlation test. Publicly available data were downloaded and analyzed for Saccharomyces cerevisiae (ref. ¹⁷), Drosophila melanogaster (GSE47281 and SRR1197331), Mus musculus (ref. ¹⁸), Homo sapiens (refs. ^19,20), and Arabidopsis thaliana (ref. ²¹). One replicate of ChIP-seq data from each sample was selected for analysis.