Fig. 5: The tandem PHD finger domain of Ehd3 homologs recognize H3K4me1 in land plants.
From: H3K4me1 directs H3K36me2 and H3K36me3 deposition in land plants
a Neighbor-joining dendrogram (left) and protein domain architecture (right) of the tandem plant homeodomain (PHD) domain-containing proteins in higher eukaryotes. The dendrogram was constructed based on the Ehd3 tandem PHD sequence alignment. The diverse protein domains are displayed in different colored boxes as indicated. Mm Mus musculus, Pd Phyllostomus discolor, Fc Felis catus, An Aotus nancymaae, Hs Homo sapiens, Ec Elysia chlorotica, Ob Octopus bimaculoides, Pv Penaeus vannamei, Zm Zea mays, Si Setaria italica, Os Oryza sativa, Ta Triticum aestivum, Bd Brachypodium distachyon, Ac Arabian coffee, Ca Capsicum annuum, Dc Daucus carota, Gm Glycine max, Rc Rosa chinensis, At Arabidopsis thaliana, Pp Physcomitrium patens, Cr Chlamydomonas reinhardtii. UniProt (https://www.uniprot.org) entries of the proteins are marked behind the species abbreviations. Structure superimposition of the tandem PHD in A0A1D6LDK6 (b) A0A3B6RKR3 (c) AtEHD3 (d) A0A162A0W2 (e) with Ehd3. The structure of Ehd3 is colored in cyan. f ITC results showing the binding affinities of homologous tandem PHD in Chlamydomonas reinhardtii, Physcomitrium patens, Arabidopsis thaliana, and Mus musculus with H3K4me0 and methylated H3K4 peptides. NDB no detectable binding. Source data are provided.
