Fig. 2: Hepatocyte-specific overexpression of GCN5 promotes lipid accumulation and MASLD.
From: GCN5 drives MASLD progression through LXRα/SREBP1c signaling pathway–mediated de novo lipogenesis
A Scheme for the generation of hepatocyte-specific Kat2a-overexpressing mice injected with AAV-Kat2a WT or empty AAV-LacZ via tail vein. B Successful overexpression of GCN5 protein in the liver was verified by western blot (n = 3 independent mice). C Liver weight (LW) and LW-to-body weight ratio (LW/BW) of AAV-Kat2a WT, and AAV-LacZ mice (n = 6 independent mice). D Representative H&E and Oil Red O staining of the liver. Scale bar: 50 μm. E The levels of serum TG, TC, HDL-c, and LDL-c (n = 6 independent mice). F The contents of liver TG and TC (n = 6 independent mice). G The levels of serum AST and ALT (n = 6 independent mice). H Scheme for the generation of hepatocyte-specific Kat2a-overexpressing mice injected with AAV-Kat2a Mutant (E575Q) or empty AAV-LacZ via the tail vein. I Successful overexpression of GCN5 protein in the liver was verified by western blot (n = 3 independent mice). J Liver weight (LW) and LW-to-body weight ratio (LW/BW) of AAV-Kat2a Mutant (E575Q) and AAV-LacZ mice (n = 10 independent mice). K Representative H&E and Oil Red O staining of the liver (n = 10 independent mice). Scale bar: 50 μm. L The levels of serum TG, TC, HDL-c, and LDL-c (n = 10 independent mice). M The contents of liver TG and TC (n = 10 independent mice). N The levels of serum AST and ALT (n = 10 independent mice). O–T Hepatocytes were transfected with KAT2A WT and KAT2A Mutant (E575Q) plasmids for 12 h, and then the cells were incubated with PA (200 μM) and OA (400 μM) for 24 h. The cellular TG and TC levels were detected in HL-7702 (O) HepG2 (Q) and mouse primary hepatocytes (S) (n = 3 independent cultures). The lipids were stained with Nile Red and BODIPY 493/503 (Bodipy) in HL-7702 (P) HepG2 (R) and mouse primary hepatocytes (T) (n = 6 independent cultures), scale bar: 20 μm. Error bars are represented as mean ± SEM. Statistical analysis was done using the two-tailed Student’s t test (A–N) and one-way ANOVA with Dunnett’s post-test (O–T). Source data are provided as a Source Data file.
