Fig. 5: Adipose-specific GSNOR overexpression promotes adipose tissue whitening. | Nature Communications

Fig. 5: Adipose-specific GSNOR overexpression promotes adipose tissue whitening.

From: S-nitrosoglutathione reductase GSNOR drives age-related obesity by promoting adipose tissue whitening through de-nitrosation of Beclin-1

Fig. 5: Adipose-specific GSNOR overexpression promotes adipose tissue whitening.The alternative text for this image may have been generated using AI.

a Immunoblotting for TIM23 protein levels in iWAT from 3-month-old male control and KI mice (n = 4 biological replicates). b Experimental timeline for the collection of iWAT following treatment with rosiglitazone or cold exposure. c Immunoblotting for UCP1 protein levels in iWAT from 3-month-old male control and KI mice at day 0 (DMSO: n = 2, rosiglitazone: Control n = 6, KI n = 7). d, e UCP1 protein levels (n = 5) and Ucp1 mRNA levels (WT n = 3, KI n = 4) in iWAT from 3-month-old male control and KI mice at day 15 after rosiglitazone withdraw. f Immunofluorescence staining for TOM20 protein in iWAT from 3-month-old male control and KI mice at 15 days of rosiglitazone withdrawal (n = 5 biological replicates). Scale bar, 20 μm. Data are represented as mean ± SD; Significance was assessed by Unpaired two-tailed Student’s t test (a, c, d, e, f) (*p < 0.05, ***p < 0.001, ns for not significant). iWAT inguinal white adipose tissue, KI GSNOR adipocyte knock-in. DMSO dimethyl sulfoxide.

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